کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10133457 | 1645592 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biosynthesis of poly-3-hydroxybutyrate from grass silage by a two-stage fermentation process based on an integrated biorefinery concept
ترجمه فارسی عنوان
بیوسنتز پلی-3-هیدروکسی بوتیرات از سیلوی علفزار به روش فرآیند تخمیر دو مرحله ای بر اساس یک مفهوم بیولوژیک یکپارچه
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
چکیده انگلیسی
Grass silage as a renewable feedstock for an integrated biorefinery includes nutrients and carbon sources directly available in the press juice (PJ) and in lignocellulosic saccharides from the plant framework. Here, a novel two-stage fed-batch fermentation process for biosynthesis of poly-3-hydroxybutyrate (PHB) by Cupriavidus necator DSM 531 is presented. For bacterial growth, nutrient-rich PJ was employed as a fermentation medium, without any supplements. Saccharides derived from the mechano-enzymatic hydrolysis of the press cake (PC) were subjected to a lactic acid fermentation process, before the fermentation products were fed into the polymer accumulation phase. By combination of pH-stat feeding and cell recycling, the PHB content in 22â¯gâ¯Lâ1 total-dry cells reached 39% after 32â¯h of cultivation. Using mimicked hydrolyzate of diluted PJ artificially supplemented with glucose and xylose, the resulting cell dry weight of 21â¯gâ¯Lâ1 contained 42% PHB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 269, December 2018, Pages 237-245
Journal: Bioresource Technology - Volume 269, December 2018, Pages 237-245
نویسندگان
Dominik Schwarz, Amelie K.J. Schoenenwald, Jörg Dörrstein, Jan Sterba, David Kahoun, Pavla FojtÃková, Josef VilÃmek, Doris Schieder, Cordt Zollfrank, Volker Sieber,