PRISEs (progesterone 5β-reductase and/or iridoid synthase-like 1,4-enone reductases): Catalytic and substrate promiscuity allows for realization of multiple pathways in plant metabolism

Substrate preferences of PRISEs can be altered by single amino acid mutations. Amino acids mutated in rAtSt5βR are highlighted together with the cofactor NADP+ and the substrate progesterone (in yellow).359