کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10140772 | 1646046 | 2019 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Direct and fast capture lactoferrin from cheese whey on nanoparticles of Fe3O4 combined with concanavalin A
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Ferric and ferrous were used to prepare the magnetic nanoparticles (MNPs), and concanavalin A was bound onto the MNPs as a magnetic nano-adsorbent for lactoferrin (Lf) separation. Scanning electron microscope showed that the diameter of modified MNPs was about 15.27â¯Â±â¯1.42â¯nm. The results showed that the optimum adsorption and elution conditions of modified MNPs on recovery Lf were 4â¯min and 5â¯min, respectively. The specificity of modified MNPs on recovery Lf was high, and the purity of Lf in eluent was 93.06%. The recovery rate of modified MNPs from whey and elution were more than 99.99%, and the recovery rate of Lf from whey performed a dose-dependent relationship. The maximum adsorption capacity of modified MNPs on recovery Lf was 90â¯Âµg/mg. The adsorption capacity of modified MNPs stored in phosphate buffer at 4â¯Â°C significantly higher (Pâ¯<â¯0.05) than those stored in other experimental conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 274, 15 February 2019, Pages 314-318
Journal: Food Chemistry - Volume 274, 15 February 2019, Pages 314-318
نویسندگان
J.L. Zhang, W. Di, P.M. Gong, K. Lin, L.Z. Lyu, L.W. Zhang, X. Han, Y. Ma,