کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10146430 | 1646403 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enrichment and characterization of an environmental microbial consortium displaying efficient keratinolytic activity
ترجمه فارسی عنوان
غنی سازی و مشخص کردن یک کنسرسیوم میکروبی محیطی که فعالیت کراتینولیتیک کارا را نشان می دهد
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
چکیده انگلیسی
Keratin refers to a group of insoluble and recalcitrant protein materials. Slaughterhouses produce large amount of keratinous byproducts, which are either disposed or poorly valorized through costly thermochemical processes for animal feed formulation. Learning from nature, keratinolytic microbial consortia stand as a cost-efficient and environmental friendly way to valorize this recalcitrant resource. Directed selection was applied to enrich soil-born microbial consortia, using sequential batch cultivations in keratin medium, while measuring enzymes activity and monitoring consortia compositions via 16S rRNA gene amplicon sequencing. A promising microbial consortium KMCG6, featuring mainly members of Bacteroidetes and Proteobacteria, was obtained. It possessed keratinolytic activity with <25% residual substrate remaining, which also displayed a high degradation reproducibility level after long-term cryopreservation. This work represents an advance in the field of α-keratin degradation with potential for practical applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 270, December 2018, Pages 303-310
Journal: Bioresource Technology - Volume 270, December 2018, Pages 303-310
نویسندگان
Dingrong Kang, Jakob Herschend, Waleed Abu Al-Soud, Martin Steen Mortensen, Milena Gonzalo, Samuel Jacquiod, Søren J. Sørensen,