کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10156900 | 1666435 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of different saccharides and chitin as eco-friendly additive to improve the magnetic cross-linked enzyme aggregates (CLEAs) activities
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evaluation of different saccharides and chitin as eco-friendly additive to improve the magnetic cross-linked enzyme aggregates (CLEAs) activities Evaluation of different saccharides and chitin as eco-friendly additive to improve the magnetic cross-linked enzyme aggregates (CLEAs) activities](/preview/png/10156900.png)
چکیده انگلیسی
The cross-linked enzyme aggregates (CLEAs) involves formation of a number of covalent bonds between enzyme and the matrix using glutaraldehyde. In general, amino groups of lysine, sulfhydryl groups of cysteine, phenolic OH groups of tyrosine, or imidazol group of histidine are used for enzyme binding under mild conditions. The main advantage of this method is its simplicity, economic advantages in the industrial bio catalysis. The Fe3O4 magnetic nanoparticles were synthesized by coprecipitating Fe2+and Fe3+in alkaline solution. Tannic acid was used to functionalize the Fe3O4 magnetic nanoparticles. After functionalization process, tannic acid magnetic cross-linked enzyme aggregates of enzyme (TA-MNPs-CLEAs) were prepared by cross-linking of enzyme aggregates with different saccharides as additive. The present result reported high stability, simplicity, low cost and recyclability of a saccharide-TA-MNPs-CLEAs-enzyme make it efficient as a highly active biocatalyst in biotechnological applications. The obtained results suggest that disaccharides (maltose, sucrose and lactose) and polysaccharide such as starch are eco-friendly additives to TA-MNPs-lipase and TA-MNPs-CLEAs-peroxidase and can become a powerful biocatalyst in industry applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 118, Part B, 15 October 2018, Pages 2040-2050
Journal: International Journal of Biological Macromolecules - Volume 118, Part B, 15 October 2018, Pages 2040-2050
نویسندگان
Atheer Awad Mehde, Wesen Adel Mehdi, Mahmut Ãzacar, Zeynep Ziyade Ãzacar,