کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10157373 | 1666459 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Relationship of organic mineral supplementation and spermatozoa/white blood cells mRNA in goats
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Relationship of organic mineral supplementation and spermatozoa/white blood cells mRNA in goats Relationship of organic mineral supplementation and spermatozoa/white blood cells mRNA in goats](/preview/png/10157373.png)
چکیده انگلیسی
The antioxidant properties and the protective role of organic zinc (Zn) and copper (Cu) in white blood cells (WBCs) and spermatozoa were analyzed through quantification of superoxide dismutase 1 (SOD1), catalase (CAT), glutathione peroxidase 4 (GPx4) and nuclear factor erythroid 2-like 2 (NFE2L2) and correlations were determined with sperm functional characteristics in Osmanabadi bucks. Bucks (aged 5 months; nâ=â40) were divided into ten groups, and the dietary treatments comprised of a control and nine treatment groups as follows: organic Zn as Zn 20, Zn 40 and Zn 60, organic Cu as Cu 12.5, Cu 25, Cu 37.5 and combined organic Zn and Cu as Zn 20+Cu 12.5, Zn 40+Cu 25, Zn 60+Cu 37.5, respectively per kg dry matter for a period of 8 months. The blood (120 and 240 days) and semen (240 days: 40âÃâ4â=â160) samples were collected from 40 bucks. In WBCs: the relative abundance of mRNA for SOD1, CAT, GPx4, NFE2L2 was greater (Pâ<â0.05) in (120 and 240 days) in majority of the mineral supplemented animals. In spermatozoa: the relative abundance of SOD1, NFE2L2, GPx4 and CAT mRNA was greater (Pâ<â0.05) in selected treatment groups. The abundance of SOD1 mRNA in WBCs was positively correlated (Pâ<â 0.05) with sperm mass motility (râ=â0.692, Pâ=â0.027). The abundance of GPx4 mRNA was negatively correlated (Pâ<â 0.05) with type A sperm (straightness; STR)â>â85% and amplitude of lateral head displacement (ALH) > 2.5 μm/ s) (r = â0.711, Pâ=â0.021) and (Pâ<â 0.05) positively correlated with sperm viability (râ=â0.669, Pâ=â0.035). Organic Zn and Cu supplementation was associated with an increase in the expression of antioxidant defense enzyme genes in bucks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 197, October 2018, Pages 296-304
Journal: Animal Reproduction Science - Volume 197, October 2018, Pages 296-304
نویسندگان
Arangasamy A., Renu Balkrishan Sharma, Hemalatha K., Venkata Krishnaiah M., Selvaraju S., Pushpa Rani G., Binsila B.K., Soren N.M., Reddy I.J., Ravindra J.P., Raghavendra Bhatta,