کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10158591 | 1666533 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proposed mechanism for understanding the dose- and time-dependency of the effects of fluoride in the liver
ترجمه فارسی عنوان
مکانیسم پیشنهادی برای درک تأثیر دوز و زمان بر روی فلوراید در کبد
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کلمات کلیدی
کبد، فلوراید، استرس اکسیداتیو، پروتئومیک قرار گرفتن در معرض،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
Fluoride (F) can induce changes in the expression of several liver proteins. It is suggested that these changes are dose- and time-dependent. The objective of this study was to analyze the effect of different F concentrations and exposure times to this ion on the pattern of protein expression in the liver of rats. Thirt-six 21-day-old male Wistar rats were divided into 2 groups (nâ¯=â¯18) according to the treatment duration (20 or 60â¯days). Each of these groups was then divided into 3 subgroups (nâ¯=â¯6) according to the concentration of F administered in drinking water, as follows: 0â¯mg/L (control), 15â¯mg/L or 50â¯mg/L. After the experiment periods, the animals were anesthetized and the liver and blood were collected. F was analyzed in plasma and liver. Part of the liver was fixed for histological analysis. Liver proteins were extracted and prepared for quantitative label-free mass spectrometry analysis. F concentrations in plasma and liver were significantly higher in the group treated with 50â¯mg /L in comparison with control, regardless the time of exposure. Histological alterations in the liver were more evident in the subgroups treated for 20â¯days. The proteomic analysis revealed changes in proteins related to endoplasmic reticulum and mitochondrial oxidative stress, mitochondrial alteration, apoptosis and cellular respiration upon exposure to F. The results reinforce previous findings showing that the effects of F in the liver are dose- and time-dependent and provide the molecular basis for understanding the evolution of these effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 358, 1 November 2018, Pages 68-75
Journal: Toxicology and Applied Pharmacology - Volume 358, 1 November 2018, Pages 68-75
نویسندگان
Heloisa Aparecida Barbosa da Silva Pereira, Aline Salgado Dionizio, Tamara Teodoro Araujo, Mileni da Silva Fernandes, Flávia Godoy Iano, MarÃlia Afonso Rabelo Buzalaf,