کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10159184 | 39 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The adipogenic potential of various extracellular matrices under the influence of an angiogenic growth factor combination in a mouse tissue engineering chamber
ترجمه فارسی عنوان
پتانسیل تخریب ماتریکس های خارج سلولی تحت تاثیر یک ترکیب فاکتور رشد آنژیوژنیک در یک محفظه مهندسی بافت موش
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The extracellular matrix (ECM) Matrigel⢠has frequently and successfully been used to generate new adipose tissue experimentally, but is unsuitable for human application. This study sought to compare the adipogenic potential of a number of alternative, biologically derived or synthetic ECMs with potential for human application, with and without growth factors and a small fat autograft. Eight groups, with six severe combined immunodeficient (SCID) mice per group, were created with bilateral chambers (silicone tubes) implanted around the epigastric vascular pedicle, with one chamber/animal containing a 5 mg fat autograft. Two animal groups were created for each of four ECMs (Matrigelâ¢, Myogel, Cymetra® and PuraMatrixâ¢) which filled the bilateral chambers. One group/ECM had no growth factors added to chambers whilst the other group had growth factors (GFs) (vascular endothelial growth factor-A (VEGF-A) plus fibroblast growth factor-2 (FGF-2) plus platelet-derived growth factor-BB (PDGF-BB)) added to both chambers. At 6 weeks, chamber tissue was morphometrically assessed for percent and absolute adipose tissue volume. Overall, the triple GF regime significantly increased percentâ and absolute# adipose tissue volume (p < 0.0005â#) compared to chambers without triple GF treatment. The fat autograft also significantly increased percent (p < 0.0005) and absolute (p < 0.011) adipose tissue volume. Cymetra® (human collagen) constructs yielded the largest total tissue and absolute adipose tissue volume. We found that the pro-angiogenic FGF-2, VEGF-A and PDGF-BB combination in ECMs of synthetic and biological origin produced an overall significantly increased adipose tissue volume at 6 weeks and may have clinical application, particularly with Cymetra.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Biomaterialia - Volume 10, Issue 5, May 2014, Pages 1907-1918
Journal: Acta Biomaterialia - Volume 10, Issue 5, May 2014, Pages 1907-1918
نویسندگان
Adrian C.H. Ting, Randall O. Craft, Jason A. Palmer, Yi-wen Gerrand, Anthony J. Penington, Wayne A. Morrison, Geraldine M. Mitchell,