کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10160009 | 64 | 2013 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preparation of an adipogenic hydrogel from subcutaneous adipose tissue
ترجمه فارسی عنوان
آماده سازی هیدروژل آیدپوژن از بافت چربی زیر جلدی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The ability to generate controlled amounts of adipose tissue would greatly ease the burden on hospitals for reconstructive surgery. We have previously shown that a tissue engineering chamber containing a vascular pedicle was capable of forming new fat; however, further refinements are required to enhance fat formation. The development and maintenance of engineered adipose tissue requires a suitable source of growth factors and a suitable scaffold. A hydrogel derived from adipose tissue may fulfil this need. Subcutaneous fat was processed into a thermosensitive hydrogel we refer to as adipose-derived matrix (ADM). Protein analysis revealed high levels of basement membrane proteins, collagens and detectable levels of growth factors. Adipose-derived stem cells exposed to this hydrogel differentiated into adipocytes with >Â 90% efficiency and in vivo testing in rats showed significant signs of adipogenesis after 8Â weeks. ADM's adipogenic properties combined with its simple gelation, relatively long shelf life and its tolerance to multiple freeze-thaw cycles, makes it a promising candidate for adipose engineering applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Biomaterialia - Volume 9, Issue 3, March 2013, Pages 5609-5620
Journal: Acta Biomaterialia - Volume 9, Issue 3, March 2013, Pages 5609-5620
نویسندگان
Christopher J. Poon, Maria V. Pereira E. Cotta, Shiba Sinha, Jason A. Palmer, Alan A. Woods, Wayne A. Morrison, Keren M. Abberton,