Cytoplasmic production of soluble and functional single-chain Fv-Fc fusion protein in Escherichia coli

▶ Soluble and functional scFv-Fc is expressed in E. coli trxB/gor double mutant. ▶ Solubility of scFv-Fc in the cytoplasm is improved by co-expression of GroELS. ▶ Productivity of more than 10 mg/L is achieved in shake-flask culture.