Oriented adsorptive immobilization of esterase BioH based on protein structure analysis

▶ Analysis of the interaction between protein surface structure and support surface facilitates the successful adsorption-depended enzyme immobilization. ▶ Substrate adsorption on the immobilization support changes the apparent activity of immobilized enzyme. ▶ Esterase BioH successfully immobilized on pro-hydrophilic polystyrene.