کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10160637 | 242 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel method for the measurement of oxygen mass transfer rates in small-scale vessels
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel method for the measurement of oxygen mass transfer rates in small-scale vessels A novel method for the measurement of oxygen mass transfer rates in small-scale vessels](/preview/png/10160637.png)
چکیده انگلیسی
A novel method for the measurement of the volumetric oxygen transfer coefficient, kLa, using the catechol-2,3-dioxygenase (XylE) bio-oxidation of catechol yielding 2-hydroxymuconic semialdehyde (2-HS), has been developed for small-scale systems. This method was kinetically characterized and validated by comparison to other established techniques. The bio-oxidation rate was found to be zero order over a catechol concentration from 2 to 7 mM lâ1. When the enzyme concentration was in excess and the bio-oxidation rate was mass transfer limited, indicated by a percent DOT of zero, the oxygen transfer rate was determined from the linear increase in product concentration. The method was validated in a 2 l stirred tank vessel equipped with a DOT probe and connected to a gas mass spectrometer. The novel method yielded similar kLa values when compared to the dynamic gassing out method under the same conditions. The applicability of this novel method for small-scale devices was demonstrated by measuring kLa values of up to 150 hâ1 in shaken microplates with a working volume of only 200 μl.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 25, Issue 1, August 2005, Pages 63-68
Journal: Biochemical Engineering Journal - Volume 25, Issue 1, August 2005, Pages 63-68
نویسندگان
Kenny Ortiz-Ochoa, Steven D. Doig, John M. Ward, Frank Baganz,