کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10160701 | 254 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Insect cells-Baculovirus system: Factors affecting growth and low MOI infection
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Insect cells-Baculovirus system: Factors affecting growth and low MOI infection Insect cells-Baculovirus system: Factors affecting growth and low MOI infection](/preview/png/10160701.png)
چکیده انگلیسی
The use of low multiplicity of infection (MOI) for the production of baculovirus in insect cell sytems is an attractive alternative for large-scale production processes. Such processes may be focused on the production of biological insecticides or on the expression of medically useful foreign genes. In the present study, the effect of MOI, cell inoculum age and inoculation procedure (carry-over of spent medium from the pre-culture) was studied. While some influences of inoculum age and medium were found, the overhelming effect is related to MOI. The data obtained describe the dynamics of the changes of non-occluded virus (NOV) in time, giving a useful insight into the mechanism of the cell-virus system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 27, Issue 1, 1 December 2005, Pages 8-16
Journal: Biochemical Engineering Journal - Volume 27, Issue 1, 1 December 2005, Pages 8-16
نویسندگان
You H. Zhang, Giora Enden, José C. Merchuk,