کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10160715 | 254 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Manipulation of morphological transition in Saccharomyces cerevisiae mutant J19
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Manipulation of morphological transition in Saccharomyces cerevisiae mutant J19 Manipulation of morphological transition in Saccharomyces cerevisiae mutant J19](/preview/png/10160715.png)
چکیده انگلیسی
Saccharomyces cerevisiae has the potential for morphological differentiation. In order to positively utilize the morphological transition of S. cerevisiae for the separation of cells from culture broth, the cell settling of mutant J19 having pseudohyphal form was examined. Mutant cells settled faster in stationary test tubes than those of the parental strain having yeast-like form. Furthermore, in an attempt to establish a basis for manipulation of the morphological transition between the yeast-like and pseudohyphal forms, a plasmid harboring the SCL1 gene under the control of the GAL1 promoter was constructed. The SCL1 gene complements the morphological phenotype of mutant J19. The plasmid was transformed into mutant J19, and the morphological transition of the transformant by changes in carbon source was examined. It was found that partial manipulation of the morphological transition was possible.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 27, Issue 1, 1 December 2005, Pages 72-76
Journal: Biochemical Engineering Journal - Volume 27, Issue 1, 1 December 2005, Pages 72-76
نویسندگان
Jaruwan Maneesri, Masayuki Azuma, Koichi Igarashi, Akio Sugihara, Hiroshi Ooshima,