کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10160831 | 268 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Repeated elicitation enhances phenylethanoid glycosides accumulation in cell suspension cultures of Cistanche deserticola
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
Effect of yeast elicitor on growth and phenylethanoid glycosides (PeGs) accumulation in Cistanche deserticola cell suspension cultures was investigated. Both biomass and PeGs production were enhanced by the addition of yeast elicitor. The dry weight of 9.0 g lâ1 and PeGs production of 237.7 mg lâ1 in 21-day cell cultures treated with 106 mg total sugar lâ1 medium yeast elicitor for 3 days were achieved. Furthermore, a strategy of repeated addition of yeast elicitor was developed. Yeast elicitor repeatedly added on days 15, 17 and 19 at the dose of 106 mg total sugar lâ1 medium improved PeGs accumulation further and the final PeGs production in the cell cultures of C. deserticola reached 317.8 mg lâ1. The enhanced PeGs accumulation in C. deserticola cell suspension cultures was related to the increase of phenylalanine ammonium lyase activity stimulated by yeast elicitor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 24, Issue 3, July 2005, Pages 203-207
Journal: Biochemical Engineering Journal - Volume 24, Issue 3, July 2005, Pages 203-207
نویسندگان
Xi-Yu Cheng, Bin Guo, Hua-Ying Zhou, Wen Ni, Chun-Zhao Liu,