کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10179959 | 1318695 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reference genes for quantitative real-time PCR analysis and quantitative expression of P5CS in Agropyron mongolicum under drought stress
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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چکیده انگلیسی
Reference genes, stably expressing in different tissues and cells, are commonly used as the references in expression analysis. Selecting the optimum reference gene is crucial to the success of experiments. In this study, the expression stabilities of nine common reference genes, including ACT2, 18S rRNA, APRT, EF-1α, RNA POL II, TUBα, TUBβ, GAPDH and TLF of Agropyron mongolicum, were studied under drought condition. Among them, 18S rRNA was found to be the most optimum reference gene under drought stress by the analyzing of geNorm and NormFinder software. Quantitative expression levels of P5CS using 18S rRNA as the reference gene, and proline contents under drought stress in A. mongolicum were further operated, and we found the expression level of P5CS gene and proline content had a significantly positive relationship (R2=0.7763, P<0.05). This study established and validated 18S rRNA as the reference genes in A. mongolicum under drought stress, providing a powerful tool for the quantitative expression analysis of drought genes in A. mongolicum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Integrative Agriculture - Volume 15, Issue 9, September 2016, Pages 2097-2104
Journal: Journal of Integrative Agriculture - Volume 15, Issue 9, September 2016, Pages 2097-2104
نویسندگان
Qing-song TIAN, Shu-yan WANG, Jian-cai DU, Zhi-juan WU, Xiao-quan LI, Bing HAN,