کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10227458 446 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polyion complex micellar nanoparticles for integrated fluorometric detection and bacteria inhibition in aqueous media
ترجمه فارسی عنوان
پلی یون نانو ذرات مایکرولا پیچیده برای تشخیص یکپارچه فلورومتریک و مهار باکتری ها در محیط های آبی
کلمات کلیدی
پلی ایزیته مکزیکی پیچیده، انتشار ناشی از انباشته شدن، تشخیص باکتری، ضد میکروبی انسولین مولکولی، بیوسنسورها،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
The development of portable and inexpensive detection methods can significantly contribute to the prevention of water-borne infectious diseases caused by pathogenic bacteria. Here we designed a nanosystem capable of both bacterial detection and inhibition, where polyion complex (PIC) micelles are constructed from negatively-charged tetraphenylethylene (TPE) sulfonate derivatives, which exhibit the aggregation-induced emission (AIE) feature, and cationic diblock copolymers, poly(ethylene oxide)-b-quaternized poly(2-(dimethylamino)ethyl methacrylate) (PEO-b-PQDMA). Upon contacting with bacteria, the PIC nanosystem disintegrates presumably due to competitive binding of polycation blocks with negatively-charged bacterial surfaces. This process is accompanied by a conspicuous quenching of TPE fluorescence emission, serving as a real-time module for microbial detection. Furthermore, the sharp decrease in CFU is indicative of prominent anti-microbial activities. Thus, PIC micelles possess dual functions of fluorometric detection and inhibition for bacteria in aqueous media. By tuning the charge density of TPE sulfonate derivatives and chain length of cationic PQDMA blocks, optimal performance against Gram-negative Escherichia coli has been achieved with a detection limit of 5.5 × 104 CFU/mL and minimum inhibitory concentration (MIC) of 19.7 μg/mL. Tests against Gram-positive Staphylococcus aureus were also conducted to demonstrate versatility of the nanosystem.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 5, February 2014, Pages 1618-1626
نویسندگان
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