کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10227609 451 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells
ترجمه فارسی عنوان
زیربنای ژلاتین نانوفیبریل برای رشد طولانی مدت سلول های بنیادی پلوروپتوپ انسانی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Nanofibrous gelatin substrates are suited for long-term expansion of human pluripotent stem cells (hPSCs) under feeder- and serum-free culture conditions. A combinatorial library with different sets of processing parameters was established to assess the culture performance of hPSCs on nanofibrous substrates in terms of cell adhesion and growth rate, using Matrigel as control. Then, the optimal conditions were applied to long-term expansion of hPSCs with several cell lines, showing a maintained pluripotency over more than 20 passages without introducing any abnormal chromosome. In addition, this approach allowed us to avoid enzymatic disassociation and mechanic cutting during passages, thereby promoting a better hPSC culture and long-term expansion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 24, August 2014, Pages 6259-6267
نویسندگان
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