کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10227908 465 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tube length and cell type-dependent cellular responses to ultra-short single-walled carbon nanotube
ترجمه فارسی عنوان
طول لوله و پاسخ سلولی وابسته به نوع سلول به نانولوله های کربنی تک سیال فوق العاده کوتاه
کلمات کلیدی
نانولوله کربنی تک دیواره، اثر اندازه، وابسته به نوع سلول، قاچاق داخل سلولی، انتقال نسل،
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
This paper presents a detailed study on the cellular responses to PEGylated ultra-short (<80 nm) single-walled carbon nanotube (US-SWNT). The experimental results show clearly the tube length and cell-type dependent cellular uptake, intracellular localization, excretion of US-SWNT, as well as US-SWNT partitioning at cell division. Confocal fluorescence imaging and flow cytometry analysis of three cell types (HeLa, human hepatoma, and HUVEC) indicate that PEGylated SWNT below 35 nm might not be suitable for active targeting but could find alternative applications in gene transfection due to the ability to spontaneously traverse the nuclear membrane. While US-SWNT with an average length of 30 nm were rapidly excreted by non-polarized HeLa and hepatoma cells, lysosomal retention was observed by HUVEC, a polarized cell line. Further, HUVEC transferred intracellular US-SWNT to subsequent generations through asymmetric partitioning. These results could have significant implications for the rational design of SWNT carriers for drug delivery, as contrast agents, and for other new niche applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 35, Issue 9, March 2014, Pages 3121-3131
نویسندگان
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