کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10228221 | 475 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intracellular micro-rheology probed by micron-sized wires
ترجمه فارسی عنوان
میکرو رولوژی داخل سلولی مورد بررسی قرار گرفته توسط سیم های میکرو اندازه
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کلمات کلیدی
سیم مغناطیسی، سلول های زنده، داخلی سازی، رئوئولوژی، میکرو رولوژیک منفعل، محیط داخل سلولی،
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
In the last decade, rapid advances have been made in the field of micro-rheology of cells and tissues. Given the complexity of living systems, there is a need for the development of new types of nano- and micron-sized probes, and in particular of probes with controlled interactions with the surrounding medium. In the present paper, we evaluate the use of micron-sized wires as potential probes of the mechanical properties of cells. The wire-based micro-rheology technique is applied to living cells such as murine fibroblasts and canine kidney epithelial cells. The mean-squared angular displacement of wires associated to their rotational dynamics is obtained as a function of the time using optical microscopy and image processing. It reveals a Brownian-like diffusive regime of the form ãÎÏ2(t,L)ãâ¼t/L3, where L denotes the wire length. This scaling suggests that an effective viscosity of the intracellular medium can be determined, and that in the range 1-10 μm it does not depend on the length scale over which it is measured.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 27, September 2013, Pages 6299-6305
Journal: Biomaterials - Volume 34, Issue 27, September 2013, Pages 6299-6305
نویسندگان
Loudjy Chevry, Rémy Colin, Bérengère Abou, Jean-François Berret,