کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10230091 | 673 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polyampholytes as low toxic efficient cryoprotective agents with antifreeze protein properties
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
Dimethyl sulfoxide (DMSO) has been used for several decades as the most efficient cryoprotective agent (CPA) for many types of cells and tissues in spite of its cytotoxicity and its effects on differentiation. Here we report that polyampholytes with an appropriate ratio of amino and carboxyl groups show higher cryopreservation efficiency and lower cytotoxicity than DMSO. Culture medium solutions of É-poly-l-lysine (PLL) with more than 50Â mol% of amino groups carboxylated showed excellent post-thaw survival efficiency of 95% murine L929 cells, and rat mesenchymal stem cells fully retained the potential for differentiation without serum. We also found that carboxylated PLLs showed antifreeze protein properties, such as ice recrystallization inhibition, which may contribute to successful cryopreservation by membrane protection. Thus, these polyampholytes can replace DMSO as new materials for CPAs in various preserving functions and will also be useful in studies elucidating the mechanisms of cryopreservation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 30, Issue 27, September 2009, Pages 4842-4849
Journal: Biomaterials - Volume 30, Issue 27, September 2009, Pages 4842-4849
نویسندگان
Kazuaki Matsumura, Suong-Hyu Hyon,