کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10231538 | 1207 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression, stabilization and purification of membrane proteins via diverse protein synthesis systems and detergents involving cell-free associated with self-assembly peptide surfactants
ترجمه فارسی عنوان
بیان، تثبیت و پاکسازی پروتئین های غشایی از طریق سیستم های مختلف سنتز پروتئین و مواد پاک کننده شامل سلول های آزاد همراه با سورفکتانت های پپتیدهای خودمراقبتی
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کلمات کلیدی
SDCConALCPDcytBMbCDGPCRsORSSf21Sample displacement chromatographyT4LMBPPS-IDDME. coliG-protein coupled receptors - G-پروتئین گیرنده های متصل شدهn-Dodecyl-β-D-maltoside - n-Dodecyl-β-D-مالتوزیدExtract - استخراج کردنEscherichia coli - اشریشیا کُلیCell-free - بدون سلولTAAR - تارCytochrome b - سیتوکروم بphotosystem I - عکس سیستم منLipid cubic phase - فاز مکعبی لیپیدtrigger factor - فاکتور مهارT4 lysozyme - لیزوزیم T4methyl-beta-cyclodextrin - متیل بتا سیکلوکودکسترینmaltose-binding protein - پروتئین متصل به مالتوزOlfactory receptors - گیرنده های التهابی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
G-protein coupled receptors (GPCRs) are involved in regulating most of physiological actions and metabolism in the bodies, which have become most frequently addressed therapeutic targets for various disorders and diseases. Purified GPCR-based drug discoveries have become routine that approaches to structural study, novel biophysical and biochemical function analyses. However, several bottlenecks that GPCR-directed drugs need to conquer the problems including overexpression, solubilization, and purification as well as stabilization. The breakthroughs are to obtain efficient protein yield and stabilize their functional conformation which are both urgently requiring of effective protein synthesis system methods and optimal surfactants. Cell-free protein synthesis system is superior to the high yields and post-translation modifications, and early signs of self-assembly peptide detergents also emerged to superiority in purification of membrane proteins. We herein focus several predominant protein synthesis systems and surfactants involving the novel peptide detergents, and uncover the advantages of cell-free protein synthesis system with self-assembling peptide detergents in purification of functional GPCRs. This review is useful to further study in membrane proteins as well as the new drug exploration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biotechnology Advances - Volume 32, Issue 3, MayâJune 2014, Pages 564-574
Journal: Biotechnology Advances - Volume 32, Issue 3, MayâJune 2014, Pages 564-574
نویسندگان
Xuan Zheng, Shuangshuang Dong, Jie Zheng, Duanhua Li, Feng Li, Zhongli Luo,