کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10233166 | 42730 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Continuous ethanol production using self-flocculating yeast in a cascade of fermentors
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Continuous ethanol fermentation using a self-flocculating yeast strain SPSC01 was examined in a four-stage tanks in series fermentation system with a total working volume of 4000 ml. The first tank was designated for seed cultivation and the others for ethanol fermentation. Two-stage enzymatic hydrolysate of corn powder containing total sugar of 120 g lâ1, supplemented with 2.0 g lâ1 (NH4)2HPO4 and K2HPO4, respectively, was used as seed culture substrate and fed into the seed fermentor at the dilution rate of 0.017 hâ1. Meanwhile, the hydrolysate containing total sugar of 220 g lâ1, supplemented with 0.5 g lâ1 (NH4)2HPO4 and 0.15 g lâ1 K2HPO4 was used as ethanol fermentation substrate and fed into the second tank at the dilution rates of 0.017, 0.025, 0.033, 0.040 and 0.050 hâ1, respectively. Steady states were observed when the fermentation system was operated at the dilution rates of 0.017, 0.025, 0.033 and 0.050 hâ1. However, when the fermentation system was operated at the dilution rate of 0.040 hâ1, unsteady states and oscillations that characterized by big fluctuations of residual sugar, ethanol and biomass were detected. Compared with freely suspended yeast cell ethanol fermentation system, this self-flocculating yeast strain could partly self-immobilize within the tanks. The effluent containing 95.6 g lâ1 ethanol, 1.5 g lâ1 reducing sugar and 2.5 g lâ1 total sugar was steadily produced when the fermentation system was operated at the dilution rate of 0.033 hâ1. The ethanol productivity of 3.44 g lâ1 hâ1 was achieved, which almost doubled that of freely suspended yeast cell system when the same levels of ethanol and residual sugars were reached. The conversion yield of ethanol to total sugar in the media was calculated to be 0.465, equivalent to 91.1% of its theoretical value of 0.511.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 37, Issue 6, 1 November 2005, Pages 634-640
Journal: Enzyme and Microbial Technology - Volume 37, Issue 6, 1 November 2005, Pages 634-640
نویسندگان
T.J. Xu, X.Q. Zhao, F.W. Bai,