A comparison of the activities of three β-galactosidases in aqueous-organic solvent mixtures

Changes in Vmax/KM for the β-galactosidase-catalysed hydrolysis of 2-nitrophenyl-β-d-galactopyranoside (ONPGal) are weakly correlated with lipophilicity of the added cosolvent. In several cases, we successfully used various organic water-miscible solvent systems in the aid of oligosaccharides versus hydrolysis.