کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10233234 42737 2005 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aldehyde-dextran-protein conjugates to immobilize amino-haptens: avoiding cross-reactions in the immunodetection
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Aldehyde-dextran-protein conjugates to immobilize amino-haptens: avoiding cross-reactions in the immunodetection
چکیده انگلیسی
Proteins covered with aldehyde-dextran have been used as carrier molecules to detect the immuno-response against an aminated hapten. These conjugates were used in immunodetection after immobilization on ELISA plates and compared to hapten-protein conjugates produced by the same chemistry that was used to induce the immuno-response (by carbodiimide coupling method). It has been found that the use of dextran-protein carriers conjugates avoid the high cross-reaction found using the carbodiimide routes. The modification degree of the dextran by aminated haptens depended on the pH (the best results were achieved at alkaline pH values) and presence of organic solvents (the presence of dioxane improved the modification).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 36, Issue 4, 2 March 2005, Pages 510-513
نویسندگان
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