کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10233244 | 42737 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Engineering of Escherichia coli fatty acid metabolism for the production of polyhydroxyalkanoates
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Engineering of Escherichia coli fatty acid metabolism for the production of polyhydroxyalkanoates Engineering of Escherichia coli fatty acid metabolism for the production of polyhydroxyalkanoates](/preview/png/10233244.png)
چکیده انگلیسی
Polyhydroxyalkanoates (PHAs) are a family of biodegradable polyesters, which are synthesized and intracellularly accumulated as carbon and energy sources in numerous microorganisms. Increased knowledge of the molecular and genetic bases of PHA biosynthesis has led to the engineering of recombinant Escherichia coli expressing heterologous PHA biosynthesis genes for production of different types of PHA. In addition, inherent E. coli metabolic pathways have been engineered to efficiently supply PHA monomers from renewable carbon sources, such as sugars and fatty acids. Efficient biosynthesis from sugars of short-chain-length (SCL) PHAs composed of monomers with three to five carbon atoms can be achieved in E. coli, with production levels reaching 5 g PHA Lâ1 hâ1. However, biosynthesis of medium-chain-length (MCL) PHAs composed of monomers having 6-14 carbon atoms, and other PHA copolymers, is problematic in E. coli due to the difficulty of drawing PHA precursors from the fatty acid metabolic pathways. In this paper, we discuss recent advances in recombinant E. coli metabolic engineering strategies for synthesis of PHA from intermediates of fatty acid metabolism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 36, Issue 4, 2 March 2005, Pages 579-588
Journal: Enzyme and Microbial Technology - Volume 36, Issue 4, 2 March 2005, Pages 579-588
نویسندگان
Si Jae Park, Jong-il Choi, Sang Yup Lee,