کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10233342 | 42748 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthesis, test and application of chirale fluorescence substrates to evaluate enzymatic processes in different reaction media
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A pseudo-enantiomeric reaction was developed which was monitored on-line via 2D-fluorescence spectroscopy. During enzymatic reactions the enantiomeric excess can be followed on-line. Fluorescence spectroscopic detectable substrates, l-/d-phenylalanine-7-amido-4-methylcoumarine (l-/d-PheAMC) and l-/d-phenylalanine-7-amido-4-trifluoro-methylcoumarine (l-/d-PheAFC) were synthesized and deployed for applications in aqueous systems. Several proteases and esterases were tested to detect suitable biocatalysts for the enzymatic hydrolysis of the coumarine substrates. With α-chymotrypsin and the esterase from porcine liver, every coumarine substrate was hydrolyzed in an aqueous system and the determined enzymatic parameters were compared. The enantioselectivity and the enantiomeric excess of the enzymatic reactions were investigated. In simultaneous applications of the l- and d-substrates, the reactions were monitored on-line and the enantioselectivities, enantiomeric excesses and kinetic parameters were examined. To display the versatility of the on-line method, the enzymatic reactions were transferred to the organic solvent toluene. After the optimisation of the biocatalysis in toluene, the kinetic parameters were measured and the ee-parameters for the substrates and products were examined in off-line and on-line investigations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 37, Issue 7, 1 December 2005, Pages 673-686
Journal: Enzyme and Microbial Technology - Volume 37, Issue 7, 1 December 2005, Pages 673-686
نویسندگان
Torsten Knüttel, Hartmut Meyer, Thomas Scheper,