کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10233432 | 42752 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hydrolytic resolution of (R,S)-ethyl mandelate in biphasic media via Klebsiella oxytoca hydrolase
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
A thermally stable Klebsiella oxytoca hydrolase was explored as an enantioselective biocatalyst for the hydrolytic resolution of (R,S)-ethyl mandelate in biphasic media. Effects of various process parameters such as solvent type, temperature, pH, product inhibition, enzyme loading and substrate concentration on the enzyme performance were studied, leading to the high enzyme (S)-enantioselectivity of E = 56 at 45 °C for the reaction media consisting of iso-octane and pH 7 buffer. The optimal conditions of pH between 7 and 8, temperature 45 °C and enzyme loading no less than 0.5 mg/ml were proposed for obtaining optically pure (R)-ethyl mandelate remained in the organic phase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 37, Issue 2, 1 July 2005, Pages 266-271
Journal: Enzyme and Microbial Technology - Volume 37, Issue 2, 1 July 2005, Pages 266-271
نویسندگان
Pei-Yun Wang, Shau-Wei Tsai,