کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10233432 42752 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hydrolytic resolution of (R,S)-ethyl mandelate in biphasic media via Klebsiella oxytoca hydrolase
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Hydrolytic resolution of (R,S)-ethyl mandelate in biphasic media via Klebsiella oxytoca hydrolase
چکیده انگلیسی
A thermally stable Klebsiella oxytoca hydrolase was explored as an enantioselective biocatalyst for the hydrolytic resolution of (R,S)-ethyl mandelate in biphasic media. Effects of various process parameters such as solvent type, temperature, pH, product inhibition, enzyme loading and substrate concentration on the enzyme performance were studied, leading to the high enzyme (S)-enantioselectivity of E = 56 at 45 °C for the reaction media consisting of iso-octane and pH 7 buffer. The optimal conditions of pH between 7 and 8, temperature 45 °C and enzyme loading no less than 0.5 mg/ml were proposed for obtaining optically pure (R)-ethyl mandelate remained in the organic phase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 37, Issue 2, 1 July 2005, Pages 266-271
نویسندگان
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