Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality

- 456 uncharacterised antibodies were arrayed and incubated with protein extracts.
- Some antibodies were informative indicating expression variations between samples.
- The most promising antibodies were picked for characterisation on antigen arrays.
- The affinity of specific molecules was determined by surface plasmon resonance.
- Good binders were used to confirm the biological result by immunohistochemistry.