کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10234981 44961 2014 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Towards improved membrane protein production in Pichia pastoris: General and specific transcriptional response to membrane protein overexpression
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Towards improved membrane protein production in Pichia pastoris: General and specific transcriptional response to membrane protein overexpression
چکیده انگلیسی
Microarray experiments revealed varying transcriptional responses depending on the enzymatic activity, subcellular localization and physiological role of the membrane proteins. While an alternative oxidase evoked primarily a response within the mitochondria, the overexpression of transporters entering the secretory pathway had a wide effect on lipid metabolism and induced the upregulation of the UPR (unfolded protein response) transcription factor Hac1p. Coexpression of P. pastoris endogenous HAC1 increased the levels of ER-resident membrane proteins 1.5- to 2.1-fold. Subsequent transcriptome analysis of HAC1 coexpression revealed an upregulation of the folding machinery correlating with an expansion of the ER membrane capacity, thus boosting membrane protein production. Hence, our study has helped to elucidate the cellular response of P. pastoris to the expression of different classes of membrane proteins and led specifically to new insights into the effect of PpHac1p on membrane proteins entering the secretory pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 31, Issue 6, 25 December 2014, Pages 538-552
نویسندگان
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