Molecular interference in antibody-antigen interaction studied with magnetic force immunoassay

- We used magnetic tweezers to apply forces to proteins in a sandwich immunoassay format.
- The force-induced dissociation rate was quantified in a cTn sandwich immunoassay.
- Interference of Pluronic F-127, a non-ionic surfactant, was studied.
- The surfactant increases the dissociation rate already at low concentrations.
- Circular dichroism data show that the surfactant influences the conformation of cTn.