کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10234998 44962 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Highly specific DNA detection employing ligation on suspension bead array readout
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Highly specific DNA detection employing ligation on suspension bead array readout
چکیده انگلیسی
We show for the first time that monomerized rolling circle amplification (RCA) products can be directly detected with the Luminex suspension bead array readout without the need of PCR amplification. Furthermore, using monomerized RCA products to guide ligation of the detection oligonucleotide (DO) to barcode sequences on the magnetic Luminex beads, combined with efficient washing and increased measurement temperature, yields a higher signal to noise ratio. As a proof-of-principle, we demonstrate detection of pathogenic DNA sequences with high reproducibility, sensitivity and a dynamic range over four orders of magnitude. Using padlock probes in combination with bead suspension arrays opens up the possibility for highly multiplexed DNA targeting and readout.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 32, Issue 5, 25 September 2015, Pages 504-510
نویسندگان
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