کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10235001 44963 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative determination of two probiotics by QCM and OWLS-based immunosensors
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Comparative determination of two probiotics by QCM and OWLS-based immunosensors
چکیده انگلیسی
Sulfo-LC-SPDP cross linking agent was used to bind antibodies to the gold surface of the QCM's AT-cut quartz wafer. Concerning OWLS, antibodies were covalently bound to the amino groups of the silanized surface of the waveguide by glutaraldehyde. The dynamic measuring range was found between 1.0E+3 and 5.0E+5 CFU mL−1 in 100 fold diluted fermented milk products by QCM and with OWLS. Considering the current legislation of the probiotic content in probiotic products, the two developed immunosensors can be applied for rapid quantification of L. acidophilus and B. bifidum in fermented milk. These examinations offer effective alternatives to the microbiological plate counting method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 31, Issue 5, 25 September 2014, Pages 395-401
نویسندگان
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