کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10235009 | 44963 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The influence of shear on the metabolite yield of Lactobacillus rhamnosus biofilms
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A tubular recycle bioreactor was employed to ensure homogeneous shear conditions on the biofilm surface. Superficial liquid velocities of 0.19 m sâ1, 0.37 m sâ1, 0.55 m sâ1 and 3.65 m sâ1 were used. The highest velocity resulted in negligible cell attachment (chemostat) while the ratio of attached-to-total cell mass escalated as the superficial velocity decreased. The lactic acid yield on glucose increased from 0.75 g gâ1 to 0.90 g gâ1 with declining shear while the corresponding acetoin yield on glucose decreased from 0.074 g gâ1 to 0.017 g gâ1. Redox analysis of the catabolites revealed a net consumption of NADH in the anabolism, while the extent of NADH consumption decreased when shear was reduced. This was attributed to the formation of more extracellular polymeric substance (EPS) at low shear conditions. A simplified metabolic flux model was used to estimate the EPS content of the biomass as a function of the shear velocity. Rate data supported the notion of increased EPS at lower shear.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 31, Issue 5, 25 September 2014, Pages 460-467
Journal: New Biotechnology - Volume 31, Issue 5, 25 September 2014, Pages 460-467
نویسندگان
Hendrik Gideon Brink, Willie Nicol,