کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10235074 44974 2012 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation
چکیده انگلیسی
The generation of affinity reagents to large numbers of human proteins depends on the ability to express the target proteins as high-quality antigens. The Structural Genomics Consortium (SGC) focuses on the production and structure determination of human proteins. In a 7-year period, the SGC has deposited crystal structures of >800 human protein domains, and has additionally expressed and purified a similar number of protein domains that have not yet been crystallised. The targets include a diversity of protein domains, with an attempt to provide high coverage of protein families. The family approach provides an excellent basis for characterising the selectivity of affinity reagents. We present a summary of the approaches used to generate purified human proteins or protein domains, a test case demonstrating the ability to rapidly generate new proteins, and an optimisation study on the modification of >70 proteins by biotinylation in vivo. These results provide a unique synergy between large-scale structural projects and the recent efforts to produce a wide coverage of affinity reagents to the human proteome.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 29, Issue 5, 15 June 2012, Pages 515-525
نویسندگان
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