Selection of downstream steps by analysis of protein surface property: A case study for recombinant human lactoferrin purification from milk of transgenic cow

- Analysis of protein surface property was used for rational selection of downstream steps of proteins.
- Downstream steps for purification of rHLF from milk of transgenic cow were selected.
- Casein was removed by calcium precipitation leaving rHLF in the supernatant.
- One step cationic ion exchange chromatography realized complete separation of rHLF from BLF.
- The purification process was scaled up to pilot-plant scale of 500 l milk of transgenic cow per batch.