Enhancement of human erythropoietin production in Chinese hamster ovary cells through supplementation of 30Kc19-30Kc6 fusion protein

- Improved soluble expression of 30Kc6 (expression partner) when expressed with 30Kc19 in E. coli.
- 30Kc19-30Kc6 fusion protein efficiently delivered cell-impermeable 30Kc6 cargo protein.
- Supplementation of 30Kc19-30Kc6 protein increased the viability and inhibited apoptosis.
- Mitochondrial membrane potential and ATP generation were increased in various cells.
- Specific EPO productivity was increased due to multi-functional properties of 30Kc19-30Kc6.