Cloning, purification and characterization of translationally fused protein DNA methyltransferase M•HhaI-EGFP

Design of the transcriptionally-fused protein M
- HhaI-EGFP, composed of bacterial DNA-methyltransferase M
- HhaI and enhanced green fluorescent protein (GFP) is described. The mentioned M
- HhaI-EGFP was expressed in Escherichia coli ER1821 and purified by affinity chromatography on Ni-NTA agarose. According to expectations M
- HhaI-EGFP fused protein retained significant features of corresponding original proteins: the ability to transfer methyl group to the C5 carbon atom of internal cytosine in CGCG site and absorption-emission spectral characteristics. The created transcriptionally-fused protein M
- HhaI-EGFP could be used in various experiments in molecular biology.