کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10235396 | 45036 | 2014 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymatic degradation of lignocellulosic biomass by continuous process using laccase and cellulases with the aid of scaffoldin for ethanol production
ترجمه فارسی عنوان
تخریب آنزیمی بیوماس لیگنوسلولز با فرایند مستمر با استفاده از لاکاس و سلولز با کمک داربست برای تولید اتانول
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی
Biological processes for the degradation of intractable materials are still not considered to be practical due to the slow rates of enzymatic degradation. Cellulosomes are complexed enzyme systems with great degradative potential and one of the strategies for overcoming this problem. In this study, the laccase CueO from Escherichia coli was fused to the dockerin domain of a cellulosome system and further assembled with the scaffoldin miniCbpA, forming a laccase-miniCbpA complex. Compared to the individual subunits, laccase-miniCbpA complex caused a noticeable 2.1-fold increase in enzyme activity levels and enhanced degradation of various synthetic dyes, showing an increase of approximately 1.6-fold. Also, pretreated barley straw by laccase complexes was efficiently converted to bioethanol using a cellulase producing Saccharomyces cerevisiae strain. The laccase complexes caused a 2.6-fold increase in the amount of reduced sugar with an insoluble substrate in conditions with an identical amount of enzymes. The cellulolytic yeast with the aid of laccase complexes produced 2.34Â g/L ethanol after 72Â h, indicating an increase of approximately 2.1-fold compared to fermentation without the laccase complexes. This demonstrates the feasibility of developing an efficient laccase complex based on the cellulosome and this strategy may be used to degrade recalcitrant materials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 49, Issue 8, August 2014, Pages 1266-1273
Journal: Process Biochemistry - Volume 49, Issue 8, August 2014, Pages 1266-1273
نویسندگان
Jeong Eun Hyeon, Seung Kyou You, Dae Hee Kang, Sun-Hwa Ryu, Myungkil Kim, Sung-Suk Lee, Sung Ok Han,