The stability and thermodynamic parameters of a very thermostable and calcium-independent α-amylase from a newly isolated bacterium, Anoxybacillus beppuensis TSSC-1

► The Tulsi Shyam Hot reservoir never explored. ► Amylase production and purification and characteristics at various purity levels. ► Cost effective single-step purification with high purification and yield. ► Thermodynamic analysis on the enzyme stability. ► Very high thermo stability comparable to hyperthermophilic archaea.