Cholaminchloride hydrochloride-cationized gelatin/calcium-phosphate nanoparticles as gene carriers for transgenic chicken production

► We prepared the cationzied gelatin/calcium phosphate nanoparticle as a gene carrier. ► The in vitro transfection study and biocompatibility used HeLa cell. ► We produced the transgenic chicken through directly injection. ► The injection site was through area opaca into the subgerminal cavity. ► The chicken tissue could be observed the green fluorescence protein expression.