Negative chromatography on agarose-TREN as a technique for purification of protein spiked in soybean seeds extract

► Proteins extracted from soybean were extensively adsorbed onto agarose-TREN. ► Human IgG was obtained in nonretained chromatographic fractions (negative chromatography). ► The predominance of electrostatic interactions between immobilized TREN and proteins was verified. ► Dilution of the soybean extract is necessary to avoid precipitation of proteins in the column.