Deletion combined with saturation mutagenesis of N-terminal residues in transglutaminase from Streptomyces hygroscopicus results in enhanced activity and thermostability

► The activity of transglutaminase could be enhanced by the N-terminal residues deletion. ► Deletion combined with saturation mutagenesis of N-terminal residues results in enhanced activity and thermostability. ► The amino acid (Asp) is very important for the thermostability of transglutaminase.