Effect of solid-phase chemical modification on the features of the lipase from Thermomyces lanuginosus

► TLL has been reversibly immobilized on octyl Sepharose. ► Different percentages of the carboxylic groups have been aminated. ► The effect of the amination on the electrophoretic mobility of TLL has been analyzed. ► Modification produces an increase in enzyme activity, mainly at alkaline pH values. ► Their thermal stabilities were increased by the modification, mainly at acidic pH.