The kinetic behavior of dehydrogenase enzymes in solution and immobilized onto nanostructured carbon platforms

► We have described the kinetic behavior of alcohol (ADH) and aldehyde (AldDH) dehydrogenases in solution and immobilized onto carbon platform via polyamidoamine (PAMAM) dendrimers. ► The influence of pH, K+, thiol and temperature was evaluated. ► The results showed that PAMAM dendrimers provide a good environment for the immobilization of dehydrogenase enzymes. ► The employed anchoring methodology (layer-by-layer) required very low catalyst consumption, and the anchored enzymes were quite stable over a period of approximately 30 days.