A novel immobilization method for nuclease P1 on macroporous absorbent resin with glutaraldehyde cross-linking and determination of its properties

► Nuclease P1 immobilized on macroporous absorbent resin. ► The purification of enzyme uses heat-treatment to reduce the cost for research. ► Crude enzyme used for immobilization lowers the cost for industrial applications. ► The immobilization method is simultaneous immobilization and cross-linking. ► Activation energy for immobilized enzyme was lower compared with that of free enzyme.