Extraction of tetracycline from fermentation broth using aqueous two-phase systems composed of polyethylene glycol and cholinium-based salts

► The extraction of tetracycline from a fermentation broth is evaluated. ► Aqueous two-phase systems composed of cholinium-based salts and a polymer are used. ► The recovery efficiencies from the fermented broth are higher than 80%. ► The studied systems are a viable platform for biopharmaceutical companies.