کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10236014 | 45072 | 2011 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bio-indigo production in two different fermentation systems using recombinant Escherichia coli cells harboring a flavin-containing monooxygenase gene (fmo)
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Bio-indigo was produced in 3000-L batch and 5-L continuous fermentation of a recombinant Escherichia coli DH5α harboring a novel gene encoding flavin-containing monooxygenase (FMO). Batch fermentation in a 3000-L fermenter produced 911 ± 22 mg Lâ1 of indigo from 2 g Lâ1 tryptophan as a substrate (yield 46.9%) under the following culture conditions as follows: culture temperature 30 °C, pH 7.0, agitation speed 200 rpm, and aeration 3 vvm. Sufficient oxygen (aeration rate and agitation speed) was critical for bio-indigo production. For continuous fermentation in a 5-L fermenter, the volumetric productivity was found to be 11.3 mg Lâ1 hâ1 up to 110 h (final accumulated bio-indigo was 23 g) with a constant dilution rate (D) of 0.084 hâ1 (constant feeding rate of 0.167 L hâ1 with medium containing 3 g Lâ1 tryptophan). Recombinant E. coli cells have the ability to withstand the toxicity of high concentration of accumulated indigo in batch fermentation. In continuous fermentation, the recombinant cells exhibited high plasmid stability up to 110 h, after which they lost the plasmid.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 46, Issue 3, March 2011, Pages 788-791
Journal: Process Biochemistry - Volume 46, Issue 3, March 2011, Pages 788-791
نویسندگان
Gui Hwan Han, Seong Eun Bang, Bandamaravuri Kishore Babu, Man Chang, Hyun-Jae Shin, Si Wouk Kim,