کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10236189 45140 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Response surface optimisation of lipase-catalysed esterification of glycerol and n-3 polyunsaturated fatty acids from salmon oil
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Response surface optimisation of lipase-catalysed esterification of glycerol and n-3 polyunsaturated fatty acids from salmon oil
چکیده انگلیسی
Enzymatic synthesis of acylglycerols from glycerol and n-3 fatty acid concentrate, prepared from salmon oil, was optimised by response surface methodology (RSM). Salmon oil was hydrolysed using a stereospecific sn-1, sn-3 lipase from Aspergillus oryzae and filtered on hydrophobic membrane. Acylglycerols synthesis was performed using Lipozyme IM (Novo Nordisk) under different reaction conditions according to a Doehlert experimental design. Effects of temperature, water content, glycerol/fatty acid molar ratio, and enzyme/substrate ratio were studied. Pathway maximisation indicated that an esterification degree of 97% was obtained under the following conditions: glycerol/oil 1.2, temperature 46 °C, molecular sieves 3.5 g per 100 ml and enzyme/substrate 8.1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 1, January 2005, Pages 273-279
نویسندگان
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