کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10236212 | 45140 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influence of culture conditions on the production of extra-cellular 5-aminolevulinic acid (ALA) by recombinant E. coli
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Influence of culture conditions on the production of extra-cellular 5-aminolevulinic acid (ALA) by recombinant E. coli Influence of culture conditions on the production of extra-cellular 5-aminolevulinic acid (ALA) by recombinant E. coli](/preview/png/10236212.png)
چکیده انگلیسی
Extra-cellular production of 5-aminolevulinic acid (ALA) by recombinant E. coli harboring a fusion gene hemA:egfp was investigated in shake flasks and a bioreactor with chemically defined media by varying physiological factors such as the concentrations of precursors (glycine and succinic acid), and levulinic acid (LA). Optimum concentrations of glycine and succinic acid were found to be 15 and 30Â mM, respectively. When 30Â mM LA was added to the culture medium at the end of the exponential cell growth phase, intra-cellular ALA dehydratase activity was suppressed. Some imbalances might be caused by the addition of titrants to the cultivation. Therefore, the recombinant E. coli used in this study were grown with no control over the culture pH in the beginning phase. The recombinant E.coli produced significant ALA in the initial pH ranges of 6.1 and 6.5, i.e. without the addition of titrants. IPTG induction for the expression of the fusion gene did not enhance the production of extra-cellular ALA. Repeated addition of glycine, succinic acid, and LA increased the production of extra-cellular ALA and the inhibition of intra-cellular ALA activity, with up to 1.3Â g/l ALA having been produced in the cultivation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 1, January 2005, Pages 385-394
Journal: Process Biochemistry - Volume 40, Issue 1, January 2005, Pages 385-394
نویسندگان
Sang-Yun Chung, Kook-Hwa Seo, Jong Il Rhee,